| Binding to Secreted Bone Matrix in vitro
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Author:
Date:
2014-02-20
[Abstract] This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot analysis.
[摘要] 这种方法检查蛋白质的骨基质结合能力。 使用成骨分化培养基，诱导多潜能基质细胞（MSC）在体外分化成骨细胞和分泌骨基质。 后者使用茜素红S染色证实，其检测钙化沉积物（羟基磷灰石）的存在。 这些钙沉积物用于测试蛋白质的骨结合性质。 通过蛋白质印迹分析评估与钙化沉积物的结合。
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| Isolation of Multipotent Stromal Cells from Mouse Bone Marrow
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Author:
Date:
2014-02-20
[Abstract] Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or chondrocytes upon culture in specific differentiation medium.
[摘要] 从骨髓细胞产生小鼠多潜能基质细胞（MSC）对于广泛的应用是有用的。 有效地，这些MSC可以分化成脂肪细胞，骨细胞[参见"体外与分泌的骨骼基质结合" （Tormo等人，2014）]或软骨细胞在特定分化培养基中培养。
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