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Author:
Date:
2012-07-20
[Abstract] Mammalian infection by dimorphic fungi occurs through the inhalation of asexual spores (conidia), which are phagocytosed by host pulmonary alveolar macrophages of the innate immune system. Once phagocytosed, fungal conidia germinate into the pathogenic cell type; unicellular yeast cells which divide by fission (Vanittanakom et al., 2006; Boyce et al., 2011). To investigate if mutation of a particular fungal gene affects macrophage phagocytosis or the production of yeast cells, a murine macrophage cell culture assay can be utilized. This protocol was developed for Penicillium marneffei but is applicable to most dimorphic fungi.
[摘要] 两形态真菌的哺乳动物感染通过吸入无性孢子（分生孢子）发生，所述无性孢子被先天免疫系统的宿主肺泡巨噬细胞吞噬。 一旦被吞噬，真菌分生孢子萌发成致病细胞类型; 通过裂变分裂的单细胞酵母细胞（Vanittanakom et al。，2006; Boyce et al。，2011）。 为了研究特定真菌基因的突变是否影响巨噬细胞吞噬作用或酵母细胞的产生，可以利用鼠巨噬细胞培养测定法。 该方案是为马尼埃青霉开发的，但适用于大多数二形真菌。
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