{{ 'Home' | translate }} {{'About us' | translate}} {{'Sponsors' | translate}}
 
{{'Search' | translate}}
 

TRIS ULTRAPURE

TRIS超声
Company: Thermo Fisher Scientific
Catalog#: 50-213-709
Bio-protocol()
Company-protocol()
Other protocol()
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Author:
Date:
2012-06-20
[Abstract]  One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible. Hyperhosphorylated proteins usually migrate slowlier than dephosphorylated proteins. However, not all phosphorylated proteins can be readily detected, due to sub-optimal sample preparation and electrophoresis conditions. Here, an improved method is described that can detect phosphorylation of yeast proteins ranging from 15 kD to 200 kD. The improvement in gel electrophoresis should also be applicable to mammalian culture cells. [摘要]  已经成功地使用一维聚丙烯酰胺凝胶电泳来检测蛋白质磷酸化。 这种方法非常简单和高度可重现。 高磷酸化蛋白通常比去磷酸化蛋白更慢地迁移。 然而，由于次优的样品制备和电泳条件，不是所有的磷酸化蛋白都可以容易地检测。 这里，描述了一种改进的方法，其可以检测范围从15kD至200kD的酵母蛋白质的磷酸化。 凝胶电泳的改进也应适用于哺乳动物培养细胞。

In vitro Protein Kinase Assay Using Yeast Sch9
Author:
Date:
2012-06-05
[Abstract]  This protocol will describe experimental procedures for an in vitro kinase assay of the yeast protein kinase Sch9. This protocol can be tailored to detect kinase activity of other yeast protein kinase. [摘要]  该方案将描述酵母蛋白激酶Sch9的体外激酶测定的实验程序。 此协议可以定制，以检测其他酵母蛋白激酶的激酶活性。

Comments



twitter facebook linkedin
{{'About us' | translate}}
 
{{'Contact US' | translate}}

©  Bio-protocol LLC.
Terms of Service | Copyright IP Policy