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NP-40 (10% in H2O)

NP-40(10%在H 2 O中)

Company: MBL International
Catalog#: JM-2111-100
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Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
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Date:
2012-04-05
[Abstract]  Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell cycle (propidium iodide, PI staining) of GFP positive cells vs GFP negative cells is needed. Usually simple fixation of cells with 70% EtOH for PI staining tends to quench GFP signal; paraformaldehyde (PFA) fixation before ETOH fixation could help to sustain the GFP signal. [摘要]  用GFP构建体感染哺乳动物细胞以过表达或敲低靶基因是研究和操作基因表达的最常用的方法之一。 为了确定细胞周期上的靶基因功能,需要分析GFP阳性细胞与GFP阴性细胞的细胞周期(碘化丙锭,PI染色)。 通常用70%EtOH的细胞的简单固定用于PI染色倾向于淬灭GFP信号; 多聚甲醛(PFA)固定在ETOH固定之前可能有助于维持GFP信号。

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