Fetal Bovine Serum, qualified , Canada origin
Company: Thermo Fisher Scientific
Catalog#: 12483
Product evaluation:
Porous Scaffold Seeding and Chondrogenic Differentiation of BMSC-seeded Scaffolds
Author:
Date:
2015-12-20
[Abstract]  Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects (Bornes et al., 2014). BMSCs can be seeded within porous biomaterial scaffolds that support three-dimensional cell organization, chondrogenic differentiation and extracellular matrix deposition for the creation of engineered cartilage. This protocol describes our defined methods for isolation and expansion of human and ovine BMSCs, seeding of BMSCs within ...

Binding to Secreted Bone Matrix in vitro
Author:
Date:
2014-02-20
[Abstract]  This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot ...

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow
Author:
Date:
2014-02-20
[Abstract]  Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or chondrocytes upon culture in specific differentiation medium.

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