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Fetal Bovine Serum, qualified , Canada origin

胎牛血清,合格,加拿大起源

Company: Thermo Fisher Scientific
Catalog#: 12483
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Porous Scaffold Seeding and Chondrogenic Differentiation of BMSC-seeded Scaffolds
Author:
Date:
2015-12-20
[Abstract]  Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects (Bornes et al., 2014). BMSCs can be seeded within porous biomaterial scaffolds that support three-dimensional cell organization, chondrogenic differentiation and extracellular matrix deposition for the creation of engineered cartilage. This protocol describes our defined methods for isolation and expansion of human and ovine BMSCs, seeding of BMSCs within porous scaffolds and in vitro chondrogenic differentiation (Adesida et al., 2012; Bornes et al., 2015). [摘要]  骨髓来源的间充质干细胞(BMSCs)是治疗关节软骨缺陷的有希望的细胞来源(Bornes等人,2014)。 BMSCs可以种植在支持三维细胞组织,软骨形成分化和细胞外基质沉积的多孔生物材料支架中,用于创建工程化软骨。 该方案描述了我们定义的用于分离和扩增人和绵羊BMSCs,在多孔支架内接种BMSCs和在体外软骨形成分化的方法(Adesida等人,2012; Bornes et al。,2015)。

Binding to Secreted Bone Matrix in vitro
Author:
Date:
2014-02-20
[Abstract]  This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot analysis. [摘要]  这种方法检查蛋白质的骨基质结合能力。 使用成骨分化培养基,诱导多潜能基质细胞(MSC)在体外分化成骨细胞和分泌骨基质。 后者使用茜素红S染色证实,其检测钙化沉积物(羟基磷灰石)的存在。 这些钙沉积物用于测试蛋白质的骨结合性质。 通过蛋白质印迹分析评估与钙化沉积物的结合。

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow
Author:
Date:
2014-02-20
[Abstract]  Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or chondrocytes upon culture in specific differentiation medium. [摘要]  从骨髓细胞产生小鼠多潜能基质细胞(MSC)对于广泛的应用是有用的。 有效地,这些MSC可以分化成脂肪细胞,骨细胞[参见"体外与分泌的骨骼基质结合" (Tormo等人,2014)]或软骨细胞在特定分化培养基中培养。

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