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Author:
Date:
2016-07-05
[Abstract] Xylanase (E.C. 3.2.1.8) degrades β-1, 4 xylan by cleaving β-1, 4 glycosidic linkages randomly, resulting in the generation of xylose and xylo-oligosaccharides. Xylanases are produced by organisms including fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans and insects. Xylanases present considerable industrial interest for their use in paper manufacturing, improvement of animal feed digestibility, and clarification of fruit juices. In addition, this enzyme is the component of cell wall-degrading enzymes (CWDEs) during plant–pathogen interaction. Thus, considering their various applications in plant defence and also in industry, the characterization of xylanase activity becomes an important aspect. Conventionally, xylanase activity is determined by radial gel diffusion ...
[摘要] 木聚糖酶（E.C.3.2.1.8）通过随机切割β-1,4糖苷键降解β-1,4木聚糖，导致木糖和木寡糖的产生。木聚糖酶由包括真菌，细菌，酵母，海藻，原生动物，蜗牛，甲壳类动物和昆虫的生物体产生。木聚糖酶在纸张制造，动物饲料消化率的改进和果汁澄清方面具有相当的工业利益。此外，该酶是植物 - 病原体相互作用期间细胞壁降解酶（CWDE）的组分。因此，考虑到它们在植物防御和工业中的各种应用，木聚糖酶活性的表征变成重要的方面。常规地，通过使用刚果红染色（Emami和Hack，2001）的径向凝胶扩散测定法和通过DNSA测定法测定木聚糖酶活性，DNSA测定法是木聚糖酶活性的比色法（McLauchlan等人，1999; Kutasi et al。，2001）。相比之下，使用刚果红染色的径向凝胶扩散测定是定性测定，而DNSA方法是定量测定。此外，刚果红是被2012年OSHA危害交流标准（29 CFR 1910.1200）视为危险类别1B（致癌性）和类别12（生殖毒性）的化学品。在本研究中，所提出的方法使得能够在更安全和更简单的径向凝胶扩散测定中使用乙醇沉淀进行木聚糖酶活性的定性检测。琼脂平板中的乙醇沉淀已经改变自用于检测聚丙烯酰胺凝胶中木聚糖酶活性的方法（Royer和Nakas，1990）。
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