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Suba-Seal ®

Company: Sigma-Aldrich
Catalog#: Z124613
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Quantification of Ethylene Production in Tomato Leaves Infected by Xanthomonas euvesicatoria
[Abstract]  Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogen Xanthomonas euvesicatoria (X. euvesicatoria)-elicited symptom development in tomato. This protocol describes the measurement of ethylene gas produced by tomato leaves infected with X. euvesicatoria. Infected leaflets are placed in a glass tube for 30 min without sealing. The glass tubes are then capped with a septa stopper, and incubated for an hour. A 1 ml gas sample is removed from the tube using a syringe and then injected into a gas chromatograph to quantify ethylene gas levels. This protocol will be applicable for other plants with other pathogens with ... [摘要]  乙烯是控制果实成熟,开花,叶衰老以及脱落和疾病症状发展的气态植物激素。 乙烯在细菌病原体黄单胞菌属(Xanthomonas euvesicatoria)( X。euvesicatoria )中起到关键作用 - 引起番茄中的症状发展。 该方案描述了用感染了X的番茄叶产生的乙烯气体的测量。 euvesicatoria 。 将感染的小叶放置在玻璃管中30分钟而不密封。 然后将玻璃管用隔膜塞子盖上,并孵育1小时。 使用注射器从管中移出1ml气体样品,然后注入气相色谱仪中以定量乙烯气体水平。 该协议将适用于其他具有修改的其他病原体的植物。