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Author:
Date:
2016-07-05
[Abstract] Cyanobacteria are prokaryotic organisms that perform oxygenic photosynthesis. Freshwater cyanobacteria, such as Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803, are model organisms for the study of photosynthesis, gene regulation, and biotechnological applications because they are easy to manipulate genetically. However, while studying these cyanobacteria, care has to be taken with respect to genetic heterogeneity in the establishment of gene disruptants, because these cyanobacteria contain multiple chromosomal copies per cell. Here, we describe a method for the estimation of chromosomal copy number in Synechococcus 7942. Using this method, we have recently observed that the chromosomal copy number of Synechococcus 7942 ...
[摘要] 蓝细菌是进行含氧光合作用的原核生物。 淡水蓝细菌，例如细长聚球藻PCC 7942和聚集细胞 PCC 6803是用于研究光合作用，基因调节和生物技术应用的模式生物，因为它们易于基因操作。 然而，当研究这些蓝细菌时，必须注意遗传异质性在基因破坏物的建立中，因为这些蓝细菌每个细胞含有多个染色体拷贝。 在这里，我们描述了用于估计在聚球藻7942中的染色体拷贝数的方法。使用这种方法，我们最近观察到，聚球藻7942的染色体拷贝数显着地改变 其生长阶段。 这种技术可用于研究多倍体不仅在蓝细菌，而且在其他多倍体生物。
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