| In vitro Conditions for Dark Growth and Analysis of Maize Seedlings
|
|
Author:
Date:
2020-03-20
[Abstract] Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic differences in stem and leaf development compared to light-grown plants. Dark-growth conditions require the use of highly controlled plant growth environments. Here, we provide step-by-step instructions for creating a soilless and dark plant growth environment for maize using half-strength Murashige and Skoog media solidified with agar in clear boxes that are covered in aluminum foil. The benefits of this protocol are that it does not require special dark-growth conditions and the growth media can be easily and uniformly supplemented with hormones or ...
[摘要] [摘要] 玉米为理解植物性状进行适当的生长和发芽所必需的重要的模式生物。一种生长类型，变态发生，发生在没有光照的情况下。与光照生长的植物相比，在没有光照的情况下生长的幼苗在茎和叶的发育上表现出巨大的差异。黑暗生长条件要求使用高度受控的植物生长环境。在这里，我们提供使用半强度Murashige 来为玉米创建无土和深色植物生长环境的分步说明 和Skoog培养基在透明的盒子中用琼脂固化，并用铝箔覆盖。该协议的好处在于它不需要特殊的黑暗生长条件，并且可以轻松，均匀地向生长培养基中补充激素或其他化学物质。
[背景] 无土培养基和温度受控的环境已经使用了八十多年，以产生可再生的植物生长（McClary，1940； George 等，2008）。的Murashige 和Skoog（MS）培养基，含有盐，氨基酸和维生素，是最常用的植物生长中的一个媒体（的Murashige 和Skoog，1962）。MS最初是为烟草开发的，但此后已用于种植其他植物，包括 拟南芥和玉米。与其他无土培养基（例如泥炭，珍珠岩和ver石）不同，这种生长培养基不易与植物激素反应（Best 等，2014和2017； Malik 等，2014）。因此，可以将激素添加到生长培养基中，以比较野生型和突变型生长之间的反应。
...
|
|
|
| Measuring Auxin Transport Capacity in Seedling Roots of Medicago truncatula
|
|
Author:
Date:
2016-06-20
[Abstract] Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of Medicago truncatula via an agar block. The resulting radioactivity from radiolabelled auxin uptake in the roots is measured with a liquid scintillation counter. Here, we describe the measurement of auxin transport capacity around the nodulation susceptible zone in young seedling roots of M. truncatula in response to rhizobia inoculation. Similar assays could be adapted in other plant species and to answer other biological questions.
[摘要] 生长素运输能力的测量提供了涉及植物中生长素运输的生理机制的定量数据。 这种技术很容易执行，并给出快速的结果。 将放射性标记的生长素（吲哚-3-乙酸）通过琼脂块加入到Medic藜苜蓿的根部。 使用液体闪烁计数器测量根中放射性标记的生长素摄取的最终放射性。 在这里，我们描述了生长素运输能力周围的结瘤敏感区在年轻幼苗根的测量。 truncatula 响应根瘤菌接种。 类似的测定可以适用于其他植物物种并回答其他生物学问题。
|
|
|