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Author:
Date:
2016-06-20
[Abstract] The islets of Langerhans are clusters of endocrine cells located within the pancreas. Insulin-producing beta cells are the major cell type within islets, with glucagon-producing alpha cells and somatostatin-producing delta cells the other major cell types. The beta cells are the target of immune-mediated destruction in type 1 diabetes (Graham et al., 2012). Failure of beta cell function accompanied by loss of beta cell mass is also a feature of type 2 diabetes (Wali et al., 2013). Therefore studying the biology of pancreatic islets is important to understand the pathogenesis of diabetes and to develop new therapies. Here we describe the isolation of mouse islets. This requires gentle enzymatic and mechanical digestion of the exocrine tissue and density gradient ...
[摘要] 朗格汉斯岛是位于胰腺内的内分泌细胞簇。胰岛素生成β细胞是胰岛内的主要细胞类型，产生胰高血糖素的α细胞和生长抑素生成的δ细胞是其他主要细胞类型。 β细胞是免疫介导的1型糖尿病破坏的靶标（Graham等，2012）。 β细胞功能衰竭伴有β细胞量的丧失也是2型糖尿病的特征（Wali等，2013）。因此，研究胰岛的生物学对于了解糖尿病的发病机制和开发新的疗法是重要的。这里我们描述了小鼠胰岛的分离。这需要温和的酶促和机械消化外分泌组织和密度梯度分离（Chong et al。，2004; Liu和Shapiro，1995; Thomas et al。，1998）。然后，我们将描述胰岛如何可以整体培养或分散到单个细胞中，用于各种体外和体内分析。使用该协议可靠地导致200-400个胰岛的隔离，这取决于鼠标的应变。
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