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Author:
Date:
2016-06-05
[Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various issues including experimental design for lesion analysis, dietary conditions, isolation of the aorta, staining methods, morphometry, group size, age, the location within the arterial tree, and statistical analyses are important parameters that need to be addressed to obtain robust data. Here, we provide detailed methods to quantify aorta atherosclerosis.
[摘要] 动脉粥样硬化是大和中等大小动脉的慢性炎性疾病。 载脂蛋白E缺陷（ApoE -/-/- ）小鼠用作实验模型来研究人动脉粥样硬化。 ApoE -/-/- 小鼠是组成性高脂血症并且发展类似于人噬斑的内膜斑块。 包括病变分析的实验设计，饮食条件，主动脉分离，染色方法，形态测定，组大小，年龄，动脉树内的位置和统计分析的各种问题是需要解决以获得鲁棒数据的重要参数。 在这里，我们提供详细的方法来量化主动脉粥样硬化。
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