Dulbecco's Modified Eagle's Medium High glucose with stable L-glutamine (DMEM)
Company: WISENT
Catalog#: 319-015-CL
Product evaluation:
Binding to Secreted Bone Matrix in vitro
Author:
Date:
2014-02-20
[Abstract]  This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot ...

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow
Author:
Date:
2014-02-20
[Abstract]  Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or chondrocytes upon culture in specific differentiation medium.

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