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PierceTM DTT (Dithiothreitol)

Pierce TM DTT(二硫苏糖醇)

Company: Thermo Fisher Scientific
Catalog#: 20290
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MNase Digestion for Nucleosome Mapping in Neurospora
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Date:
2016-06-05
[Abstract]  Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin without a requirement of a nuclear fractionation step. [摘要]  通过微球菌核酸酶MNase消化染色质,然后高通量测序允许我们确定核小体在基因组上的位置和占据。 在这个协议中,我们描述了MNase消化丝状真菌粗糙链孢霉染色体的优化条件,而不需要核分馏步骤。

In vitro Protein Kinase Assay Using Yeast Sch9
Author:
Date:
2012-06-05
[Abstract]  This protocol will describe experimental procedures for an in vitro kinase assay of the yeast protein kinase Sch9. This protocol can be tailored to detect kinase activity of other yeast protein kinase. [摘要]  该方案将描述酵母蛋白激酶Sch9的体外激酶测定的实验程序。 此协议可以定制,以检测其他酵母蛋白激酶的激酶活性。

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