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Author:
Date:
2016-05-20
[Abstract] The second step of the dissimilatory denitrification pathway in which nitrite (NO2-) is converted to nitric oxide (NO) is catalyzed by the enzyme nitrite reductase. Two distinct enzymes are found in nature that catalyze this reaction, and they contain different metal sites, either iron (Fe), in the form of heme, or copper (Cu) (Zumft, 1997). The Pseudomonas stutzeri (P. stutzeri) RCH2 strain used in this assay contains both an Fe and a Cu form of nitrite reductase. In this assay, total nitrite reductase activity can be measured in whole cells using fumarate or some other carbon source as an electron source by measuring the disappearance of nitrite over time (Thorgersen et al., 2015).
[摘要] 其中亚硝酸盐（NO 2 - ）被转化为一氧化氮（NO）的异化脱氮途径的第二步由亚硝酸盐还原酶催化。 在自然界中发现催化该反应的两种不同的酶，并且它们包含不同的金属位点，铁（Fe），血红素或铜（Cu）形式（Zumft，1997）。 用于该测定的 Pseudomonas stutzeri （ stutzeri ）RCH2菌株含有Fe和Cu形式的亚硝酸还原酶。 在该测定中，可以通过测量亚硝酸盐随时间的消失，使用富马酸盐或一些其它碳源作为电子源在全细胞中测量总亚硝酸盐还原酶活性（Thorgersen等人，2015）。
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