| In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling
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Author:
Date:
2016-09-20
[Abstract] Dendritic spines are the post-synaptic structures that play a central role in excitatory synaptic transmission. Developmental spinogenesis relies on a variety of stimuli such as those derived from cell-cell communication and their downstream signaling. Here, we describe an in vitro assay of dendritic spine retraction using hippocampal slice culture, in which individual neurons are sparsely and brightly labeled by the Supernova method, for the study of molecular mechanisms of spine development.
[摘要] 树突状棘是突触后结构，在兴奋性突触传播中起重要作用。 发育性的发生依赖于各种刺激物，如来自细胞 - 细胞通讯及其下游信号传导的刺激。 在这里，我们描述了使用海马片段培养的树突状脊柱退缩的体外测定，其中单个神经元被Supernova方法稀疏和明亮地标记，用于研究脊柱发育的分子机制。
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| 3D Gel Invasion Assay of Gastric Cancer Cells with Fibroblasts
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Author:
Date:
2016-05-05
[Abstract] Cancer tissue is composed of cancer cells and a large number of stromal cells including fibroblasts. In order to understand the relationship between fibroblasts and cancer cells during invasion of the stroma, 3D gel invasion assay is useful. Most tumors are associated with a biologically active type of fibroblasts known as cancer-associated fibroblasts (CAFs), which promote the invasion of cancer cells. Here, we describe the method of imaging the invasion by fluorescently labeled CAFs and gastric cancer cells in gels containing extracellular matrix. For two-color fluorescence labeling of living cells, long-chain dialkylcarbocyanines, DiO and DiI were used. This method is also applicable for studying invasion by other stromal cells and cancer cells, and for evaluation of drugs targeting ...
[摘要] 癌组织由癌细胞和大量包括成纤维细胞的基质细胞组成。 为了理解在基质侵入期间成纤维细胞和癌细胞之间的关系，3D凝胶入侵测定是有用的。 大多数肿瘤与称为癌症相关成纤维细胞（CAF）的生物活性类型的成纤维细胞相关，其促进癌细胞的侵入。 在这里，我们描述的成像入侵的荧光标记CAF和胃癌细胞在含有细胞外基质的凝胶中的方法。 对于活细胞的双色荧光标记，使用长链二烷基碳菁，DiO和DiI。 该方法也适用于研究其他基质细胞和癌细胞的侵袭，以及用于评价靶向癌症基质细胞的药物。
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