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15 ml Conical bottom Centrifuge Tubes

Falcon ® 15mL高清PP离心管,圆锥底,带圆顶密封螺帽

Company: Corning
Catalog#: 352196
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In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
Author:
Date:
2017-10-20
[Abstract]  The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. Previous studies showed mesenchymal stem/stromal cells (MSCs) could improve the survival and capillary formation capacity of ECFCs in co-culture systems. In this article, we describe a protocol for in vitro co-culture of MSCs and ECFCs to prime ECFCs for better engraftment. [摘要]  发现具有强大自我更新和从头血管形成潜力的内皮细胞集落形成细胞(ECFCs)表明,ECFC可以通过改善缺血组织的新生血管形成,成为心血管疾病治疗的优良细胞来源。 然而,移植后的移植导致了低位移植。 以前的研究显示间充质干/基质细胞(MSC)可以改善共培养系统中ECFCs的存活和毛细管形成能力。 在这篇文章中,我们描述了体外协调MSCs和ECFCs共同培养ECFC以实现更好的移植。
【背景】内皮祖细胞(EPC)被定义为能够通过血管发生过程形成新血管的细胞群。 2004年,Ingram等人鉴定了来自人脐带血的称为“内皮细胞集落形成细胞(ECFC)”的离体培养物中的特异性高度增殖的EPC群体Ingram等人,2004),并且这些细胞最近被宣布代表EPCs(Medina等人,2017)。类似的群体也可以从具有等效血管化潜力和临床相关数量的人类胎盘组织中分离(Patel等人,2013; Shafiee等人,2015) )。因此,ECFC移植已被提出作为缺血性疾病如心肌梗塞或关键性腿部缺血的治疗方法。然而,移植后的ECFCs植入物和血管生成潜力被证明是低的(Shafiee等人,2017; ...

Detection of Pathogens and Ampicillin-resistance Genes Using Multiplex Padlock Probes
Author:
Date:
2017-08-20
[Abstract]  Diagnostic assays for pathogen identification and characterization are limited either by the number of simultaneously detectable targets, which rely on multiplexing methods, or by time constraints due to cultivation-based techniques. We recently presented a 100-plex method for human pathogen characterization to identify 75 bacterial and fungal species as well as 33 clinically relevant β-lactamases (Barišić et al., 2016). By using 16S rRNA gene sequences as barcode elements in the padlock probes, and two different fluorescence channels for species and antibiotic resistance identification, we managed to cut the number of microarray probes needed by half. Consequently, we present here the protocol of an assay with a runtime of approx. 8 h and a detection limit of 105 cfu ... [摘要]  用于病原体鉴定和表征的诊断测定法由依赖于多重方法的同时可检测目标的数量或由于基于培养的技术的时间限制来限制。 我们最近提出了一种用于人类病原体鉴定的100plex方法,以鉴定75种细菌和真菌物种以及33种临床相关β-内酰胺酶(Barišić等,2016)。 通过使用16S rRNA基因序列作为挂锁探针中的条形码元件,以及用于物种和抗生素抗性鉴定的两种不同的荧光通道,我们设法将需要的微阵列探针的数量减少一半。 因此,我们在这里介绍一个运行时间约为的测定方案。 8 h,检测限为105 cfu ml-1。 正确鉴定了89%的β-内酰胺酶和93.7%的物种。
【背景】β-内酰胺酶是一类提供抗β-内酰胺抗生素的抗生素抗性基因,其结构模拟D-丙氨酰-D-丙氨酸,细菌细胞壁的一个组分,从而抑制细菌细胞壁合成。 β-内酰胺酶能够水解β内酰胺抗生素β-内酰胺环的中心成分,并使其无效(Kong et al。,2010)。今天,描述了超过1000种β-内酰胺酶,并且存在巨大的潜在环境储层(Bush,2010; Brandt等,2017)。 ...

Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
Author:
Date:
2017-08-20
[Abstract]  Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al., 2009; Gropp et al., 2012). On the other hand, teratoma-forming tumorigenicity also represents a major risk factor impeding potential clinical applications of pluripotent stem cells (Miura et al., 2009; Okano et al., 2013). Recently, we reported that iPSCs derived from naked mole-rat lack teratoma-forming tumorigenicity when engrafted into the testes of non-obese diabetic/severe combined immunodeficient (NOD/SCID) ... [摘要]  多能干细胞,如诱导多能干细胞(iPSCs)和胚胎干细胞(ESC),当移植到免疫缺陷小鼠时,形成畸胎瘤。由于畸胎瘤包含所有三个胚层(内胚层,中胚层,外胚层),畸胎瘤形成测定被广泛用作多能性的指标(Evans和Kaufman,1981; Hentze等,2009; Gropp等,2012)。另一方面,畸胎瘤形成致瘤性也是阻碍多能干细胞潜在临床应用的主要危险因素(Miura et al。,2009; Okano等,2013)。最近,我们报道了由于ES细胞表达的Ras(ERAS)和替代物,嫁接到非肥胖型糖尿病/严重联合免疫缺陷型(NOD / SCID)小鼠的睾丸中,从裸鼠睾丸衍生的iPSC缺乏畸胎瘤形成致瘤性阅读框(ARF)依赖于该物种特异性的肿瘤抑制机制(Miyawaki等,2016)。在这里,我们描述了将多能干细胞移植到NOD / SCID小鼠的睾丸中以产生用于评估多能性和致瘤性的畸胎瘤的方法。
【背景】iPSCs和ESC用于再生医学细胞移植治疗中的应用。然而,当移植到免疫缺陷小鼠中时,这些细胞形成称为含有分化组织的畸胎瘤的肿瘤。因此,其畸胎瘤形成致瘤性的风险限制了其临床应用。几项研究报道了克服畸胎瘤形成肿瘤发生风险的方法(Itakura et al。,2017; Vazquez-Martin et ...

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