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Author:
Date:
2016-01-20
[Abstract] This is an assay designed to examine the radioactive phosphorous incorporation when the molecule is being synthesized, which means that only de novo synthesized phospholipids can be detected. Thus, with this technique it is possible to detect in vitro phospholipid synthesis under different required experimental conditions respect to controls (Guido and Caputto, 1990; Ferrero et al., 2014). There are different types of lipids. Among them we can find phospholipids, which contain glycerol esterified with two fatty acyl chains and a phosphate group that can also be bound to an organic molecule that acts as “hydrophilic head”, as shown in Figure 1 for the case of phosphatidylcholine. This structure confers amphipathic properties to lipid molecules that allow them to ...
[摘要] 这是设计用于当合成分子时检查放射性磷掺入的测定，这意味着可以检测到只有新合成的磷脂。因此，利用该技术，可以在相对于对照的不同所需实验条件下检测体外磷脂合成（Guido和Caputto，1990; Ferrero等人，2014） 。有不同类型的脂质。其中我们可以找到磷脂，其含有用两个脂肪酰基链酯化的甘油和磷酸基团，磷酸基团也可以结合到充当"亲水头部"的有机分子，如图1所示的磷脂酰胆碱的情况。这种结构赋予脂质分子两亲性质，使它们形成脂质双层，使磷脂成为生物膜的主要成分。
图1。磷脂结构的代表。摘录自： http：//bio1151.nicerweb .com/Locked/media/ch05/phospholipid.html
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