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Potter-Elvehjem Tissue Grinders with PTFE Pestles

Potter-Elvehjem组织研磨机与PTFE杵

Company: VWR
Catalog#: 886000-0020
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Isolation and Purification of Murine Microglial Cells for Flow Cytometry
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Date:
2016-01-05
[Abstract]  The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery of isolated murine microglial cells prior to flow cytometry analysis. [摘要]  详细的协议是用于分离不同的细胞类型从鼠脑作为神经胶质细胞,包括小胶质细胞,使用最小化细胞死亡率的酶消化。 Percoll梯度(30%至80%)分离允许在流式细胞术分析之前分离的小鼠胶质细胞的最大恢复。

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