Advanced RPMI 1640 Medium
Company: Thermo Fisher Scientific
Catalog#: 12633020
Product evaluation:
ADCC Assay Protocol
Author:
Date:
2014-01-20
[Abstract]  Antibody-dependent cell-mediated cytotoxicity (ADCC) bridges innate and adaptive immunity, and it involves both humoral and cellular immune responses. ADCC has been found to be a main route of immune protection against viral infections and cancers in vivo. Here we developed a flow cytometry based protocol for ADCC assay using human peripheral blood mononuclear cells (PBMCs) as effector cells. Using this protocol, we determined the ADCC activity of convalescent plasma IgGs from six ...

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