| In vitro CLE Peptide Bioactivity Assay on Plant Roots
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Author:
Date:
2015-12-20
[Abstract] Plant CLAVATA3/ESR (CLE)-related proteins play diverse roles in plant growth and development including regulating the development of root meristem. The mature functional forms of CLE peptides are typically 12-13 amino acids (aa) in length that are derived from the conserved C-termini of their precursor proteins. Genes encoding small secreted peptides sharing similarity to plant CLE proteins have recently been cloned from plant-parasitic nematodes, pests that infect many important crops. It is demonstrated that exogenous application of synthetic 12-14 aa CLE peptides corresponding to the CLE domain of their precursor proteins can suppress plant root growth. This protocol is to evaluate the bioactivity of CLE peptides originated from plant-parasitic nematodes by measuring the growth of ...
[摘要] 植物CLAVATA3/ESR（CLE）相关蛋白质在植物生长和发育中发挥多种作用，包括调节根分生组织的发育。 CLE肽的成熟功能形式通常为12-13个氨基酸（aa）长度，其衍生自其前体蛋白的保守C-末端。 最近从植物寄生线虫，感染许多重要作物的害虫克隆了编码与植物CLE蛋白相似的小分泌肽的基因。 证明外源应用对应于其前体蛋白的CLE结构域的合成12-14aa CLE肽可以抑制植物根生长。 该协议是通过测量CLE肽处理后植物根的生长或根尖分生组织（RAM）的大小来评价源自植物寄生线虫的CLE肽的生物活性。 用于研究的植物包括拟南芥和马铃薯。
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| Agrobacterium tumefaciens-mediated Transformation of Walnut (Juglans regia)
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Author:
Date:
2014-10-05
[Abstract] Like many woody plant species, walnut (Juglans regia) can be difficult to genetically transform and regenerate. However, somatic embryos have been used successfully for over two decades as a target tissue for transformation and regeneration of transgenic walnut plants. Walnut somatic embryos, initiated originally from developing zygotic embryos or anther tissue, will proliferate numerous secondary embryos from single cells in the epidermal layer. These single cells in intact somatic embryos can be efficiently transformed by Agrobacterium tumefaciens (A. tumefaciens). This gene transfer system is most efficient when Agrobacterium binary vector plasmids contain a scorable maker gene (e.g. uidA) and a selectable marker gene (e.g. nptII). This ...
[摘要] 像许多木本植物物种一样，核桃（ Juglans regia ）可能难以进行遗传转化和再生。然而，体细胞胚已经成功使用超过二十年作为转基因核桃植物的转化和再生的靶组织。核桃体细胞胚，最初从发育的合子胚或花药组织开始，将从表皮层中的单个细胞增殖许多次级胚。在完整的体细胞胚中的这些单细胞可以通过根癌土壤杆菌（根瘤土壤杆菌）有效地转化（ tumefaciens ）。当农杆菌二元载体质粒含有可打分的基因（例如uidA）和选择性标记基因（例如nptII）时，该基因转移系统是最有效的。该系统应适用于从单个农杆菌感受细胞经历重复胚胎发生的任何作物。在这里我们详细描述转化体细胞胚胎的方法，使这种技术可以应用于核桃和其他木本植物物种。
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| In vitro Nematode Infection on Potato Plant
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Author:
Date:
2014-01-05
[Abstract] Potato cyst nematodes (PCNs; Globodera rostochiensis and G. pallida) are devastating pests that infect potato root. We describe an in vitro assay for PCN infection on potato plantlet in tissue culture. This method is useful for studying nematode parasitism on potato and for investigating responses of potato clones/lines to PCN infection.
[摘要] 马铃薯胞囊线虫（PCNs; Globodera rostochiensis 和 G。pallida ）是感染土豆根的毁灭性害虫。 我们描述了在组织培养的马铃薯小植株上的PCN感染的体外测定。 这种方法可用于研究马铃薯上的线虫寄生和调查马铃薯克隆/株系对PCN感染的反应。
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