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Author:
Date:
2015-12-05
[Abstract] Many therapeutic viruses, such as oncolytic viruses, vaccines, or gene therapy vectors, may be administered by the intravenous route to maximize their delivery to target tissues. Blood components, such as antibody, complement and blood cells (such as neutrophils, monocytes, T cells, B cells or platelets) may result in viral neutralization and therefore reduce the therapeutic efficacy. This protocol will describe an in vitro assay by which to test the interaction of viruses with blood components. The effect of various factors can be isolated through fractionation. While whole blood can offer the most physiologically relevant snapshot, plasma can investigate the effects of antibody in concert with complement, and heat inactivated plasma will interrogate the effect of antibody ...
[摘要] 许多治疗性病毒，例如溶瘤病毒，疫苗或基因治疗载体，可以通过静脉内途径施用以最大化它们向靶组织的递送。 血液组分，例如抗体，补体和血细胞（例如嗜中性粒细胞，单核细胞，T细胞，B细胞或血小板）可导致病毒中和并因此降低治疗功效。 该方案将描述用于测试病毒与血液组分的相互作用的体外测定。 各种因素的影响可以通过分馏分离。 尽管全血可以提供最具生理学相关性的快照，但血浆可以研究抗体与补体一起的作用，而热灭活的血浆将会询问单独的抗体的作用。
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