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Author:
Date:
2015-10-20
[Abstract] Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the reaction product(s) of starch active enzymes, which can contain α-1, 4 linked and α-1, 6 linked glucose. This was first published in Molecular Microbiology (Cockburn et al., 2015). The TLC protocol is an adapted version of that published by Robyt and Mukerjea (Robyt and Mukerjea, 1994). For a summary of the products generated by starch active enzymes see the review by Hii et al. (2012).
[摘要] 薄层色谱（TLC）是用于检测单糖通过寡糖的存在的有用技术，尽管它需要针对所分析的特定糖进行优化。 在这里我们提出一种可视化的淀粉活性酶，可以包含α-1,4连接和α-1,6连接葡萄糖的反应产物的方法。 这首次发表于Molecular Microbiology（Cockburn等人，2015年）。 TLC方案是由Robyt和Mukerjea（Robyt和Mukerjea，1994）发表的适应版本。 对于由淀粉活性酶产生的产物的概述，参见Hii等人（2012）的综述。
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