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Author:
Date:
2015-10-05
[Abstract] Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope-specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused on developing an HIV-1 vaccine, we immunized rabbits with hyperglycosylated stable core immunogens, which were designed using high-resolution structural information to elicit antibodies against the primary receptor-binding, CD4-binding site on HIV-1 gp120. Using a solid phase adsorption assay, we could map the serum antibodies to the conserved CD4-binding site, a known broadly neutralizing determinant on exterior envelope glycoprotein, gp120.
[摘要] 我们使用"微汤姆"来研究番茄果实成熟和发育机制。 "微汤姆"适合于培养和实验，由于其小尺寸10至20厘米的高度和短的生命周期3个月。 还有大量关于"Micro-Tom"的公开信息，包括EST，全长cDNA克隆和转录组数据。 "Micro-Tom"植物在水培培养基中在荧光下使用在温室或植物室中的拟南芥文化架来生长，以获得具有转录组和蛋白质组分析的可重复性的数据。...
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