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AmpliTaq® DNA Polymerase with Buffer II (3000 units/tube)

AmpliTaq DNA聚合酶与缓冲液II(3000单位/管)

Company: Thermo Fisher Scientific
Catalog#: N8080153
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TCRβ Clonotype Analysis of EBV and CMV-specific Human CD8+ T Cells
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Date:
2015-10-05
[Abstract]  This protocol describes the quantification of all expressed T-cell antigen receptor (TCR) gene products within sorted (by flow cytometry) EBV and CMV-specific memory CD8+ T-cell populations using a template-switch anchored reverse transcription polymerase chain reaction (RT-PCR). [摘要]  该方案描述了使用模板转换锚定的(通过流式细胞术)EBV和CMV特异性记忆CD8 + T细胞群体中所有表达的T细胞抗原受体(TCR)基因产物的定量 逆转录聚合酶链反应(RT-PCR)。

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