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Agarose (Broad Separation Range for DNA/RNA/Genetic Analysis Grade)

琼脂糖(DNA / RNA /遗传分析级的广泛分离范围)

Company: Thermo Fisher Scientific
Catalog#: 10688973
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TCRβ Clonotype Analysis of EBV and CMV-specific Human CD8+ T Cells
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Date:
2015-10-05
[Abstract]  This protocol describes the quantification of all expressed T-cell antigen receptor (TCR) gene products within sorted (by flow cytometry) EBV and CMV-specific memory CD8+ T-cell populations using a template-switch anchored reverse transcription polymerase chain reaction (RT-PCR). [摘要]  该方案描述了使用模板转换锚定的(通过流式细胞术)EBV和CMV特异性记忆CD8 + T细胞群体中所有表达的T细胞抗原受体(TCR)基因产物的定量 逆转录聚合酶链反应(RT-PCR)。

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