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Bio-Safe II Complete Counting Cocktail, 4 Liters

生物安全II完整计数鸡尾酒,4升

Company: RPI
Catalog#: 111195
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[14C] Glucose Cell Wall Incorporation Assay for the Estimation of Cellulose Biosynthesis
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2015-09-20
[Abstract]  Cellulose is synthesized by Cellulose Synthase A proteins at the plasma membrane using the substrate UDP glucose. Herein, we provide a detailed method for measuring the incorporation of radiolabeled glucose into the cellulose fraction of the cell wall. In this method Arabidopsis seedlings are treated for 2 h with a cellulose biosynthesis inhibitor in the presence of radiolabeled glucose, and are subsequently boiled in acetic-nitric acid to solubilize non-cellulosic material. The radiolabeled glucose detected in the insoluble fraction indicates the amount of cellulose synthesized during the experimental timeframe. The short-term nature of this method is a useful tool in determining if inhibition of cellulose biosynthesis is the herbicides primary mode of action. [摘要]  纤维素由纤维素合酶A蛋白质在质膜上使用底物UDP葡萄糖合成。 本文中,我们提供了测量放射性标记的葡萄糖到细胞壁的纤维素部分中的掺入的详细方法。 在该方法中,将拟南芥幼苗在放射性标记的葡萄糖存在下用纤维素生物合成抑制剂处理2小时,随后在乙酸 - 硝酸中煮沸以溶解非纤维素材料。 在不溶性级分中检测的放射性标记的葡萄糖表示在实验时间段期间合成的纤维素的量。 该方法的短期性质是确定纤维素生物合成的抑制是否是除草剂主要作用模式的有用工具。

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