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Author:
Date:
2015-09-05
[Abstract] Protein phosphatase 2A (PP2A) is a group of important cellular regulators in eukaryotes that dephosphorylate more than 30% of cellular proteins whose activities are turned on or off by phosphorylation. In plants, PP2A was found to regulate critical components involved in plant growth and development, and in response to biotic and abiotic stresses. Therefore, determining the PP2A activities at different developmental stages, in different tissues, or in various mutants is critical in order to understand the functions of PP2A in plants. Traditional PP2A enzyme assay uses radioactive isotope and often take days to finish. This PP2A enzyme assay described here is a method to determine PP2A activity without using radioactive materials in less than 6 h.
[摘要] 蛋白磷酸酶2A（PP2A）是一组重要的细胞调节剂在真核生物中去磷酸化超过30％的细胞蛋白质，其活性通过磷酸化打开或关闭。 在植物中，PP2A被发现调节涉及植物生长和发育以及响应生物和非生物胁迫的关键组分。 因此，为了理解PP2A在植物中的功能，确定在不同发育阶段，在不同组织或在各种突变体中的PP2A活性是至关重要的。 传统的PP2A酶测定使用放射性同位素，并且通常需要几天才能完成。 这里描述的PP2A酶测定法是在不使用放射性材料的情况下在少于6小时内测定PP2A活性的方法。
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