| In vitro Regulatory T cells Differentiation From Naïve T Cells
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Author:
Date:
2014-03-20
[Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant immune responses to foreign antigens. Remarkably, regulatory T cells have been implicated in tumor cell evasion of immune responses (Curiel et al., 2004; Zou, 2006) by suppressing T cell mediated antitumor immunity. The study of the signals that promote the differentiation of this suppressive population in the tumor microenvironment has become a central issue. Here we described a detailed method to in vitro differentiate Tregs using ...
[摘要] 在过去的几年中，表达CD4，CD25和转录因子FoxP3的调节性T细胞（Treg）的一个子集已经作为T细胞耐受性和体内平衡的关键调节剂获得了相当大的关注（Sakaguchi，2004）。 这种T细胞群特别参与维持免疫自身耐受性和控制对外来抗原的异常免疫应答。 值得注意的是，调节性T细胞通过抑制T细胞介导的抗肿瘤免疫参与了肿瘤细胞逃避免疫应答（Curiel等人，2004; Zou，2006）。 促进这种抑制群体在肿瘤微环境中的分化的信号的研究已经成为一个中心问题。 在这里，我们描述了使用来自小鼠天然T细胞的肿瘤细胞条件培养基来体外鉴别Treg的详细方法，并且基于它们的特异性标记物来鉴定它们（Dalotto-Moreno等人， >，2013）。
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| Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
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Author:
Date:
2011-11-20
[Abstract] This assay can be used for studying genes related to lymphocyte proliferation and differentiation in vitro. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells proliferate extensively, the following spin infection will have high efficiency. After infection, cells are ready for the following assay as they will be undergoing proliferation or differentiation.
[摘要] 该测定法可用于体外研究与淋巴细胞增殖和分化相关的基因。 刺激淋巴细胞增殖对于MSCV的感染效率是重要的。 在过夜培养后，如果初始细胞广泛增殖，以下自旋感染将具有高效率。 感染后，细胞准备用于以下测定，因为它们将经历增殖或分化。
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| In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
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Author:
Date:
2011-11-20
[Abstract] In vitro differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells in vitro in the presence of specific stimulatory cytokines and inhibition of cytokines that are essential for the differentiation of other lineages.
[摘要] 在T辅助细胞研究中经常使用各种谱系的辅助T细胞的体外分化。 初始CD4T细胞可以在特异性刺激性细胞因子的存在下分化成T辅助细胞的某些谱系，并抑制对其他谱系分化必不可少的细胞因子。
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