| Genomic DNA Extraction and Genotyping of Dictyochloropsis Green Algae Strains
|
|
Author:
Date:
2015-08-05
[Abstract] Dictyochloropsis is an ecologically important genus of free-living and symbiotic green algae. Representatives of this genus are horizontally transmitted among several fungi of the family Lobariaceae, thus forming photobiont-mediated guilds. This protocol is suitable for extracting DNA from algal cultures and lichen samples and for genotyping seven unlinked Dictyochloropsis reticulata microsatellite markers in a single PCR multiplex.
Figure 1. Schematic representation of the analysis pipeline
[摘要] Dictyochloropsis是一种生态上重要的自由生活和共生绿藻类。 这个属的代表在家庭龙眼科的几种真菌之间水平传播，从而形成photobiont介导的公会。 该方案适用于从藻类培养物和地衣样品中提取DNA，以及用于在单个PCR多重中对七个未连接的微卫星标记进行基因分型。
图1.分析管道的示意图
|
|
|
| High-throughput Method for Determination of Seed Paternity by Microsatellite Markers
|
|
Author:
Date:
2013-04-20
[Abstract] In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.
[摘要] 在该方案中，描述了通过微卫星标记在烟草中确定种子亲子。 然而，这不包括新标记选择/鉴定的方案，而是利用为密切相关物种N产生的标记。 tabacum （Bindler ，2007）。 这是一种高通量方案，对于技术人员来说，优化和流线化以在5天内从微生物分离（从幼苗）到通过微卫星基因型数据的亲子鉴定来处理384（96×4）种子。
|
|
|