{{'Search' | translate}}
 

DPBS, no calcium, no magnesium

DPBS,无钙,无镁

Company: Thermo Fisher Scientific
Catalog#: 14190144
Bio-protocol()
Company-protocol()
Other protocol()

Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
Author:
Date:
2018-08-05
[Abstract]  Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multi-dimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently ... [摘要]  生物膜是嵌入自生或宿主衍生的外聚合物基质中的微生物细胞的固着群落。根据表面,生物膜可以是有益的或有害的。与浮游生物相比,生物膜细胞表现出更强的抗环境威胁,化学物质,抗菌药物和宿主免疫防御能力。当处于生物膜中时,微生物细胞彼此相互作用并与表面相互作用以形成结构复杂的多维结构。目前,许多成像技术和工具可用于生物膜群落的建筑分析。这允许通过获取可以呈现无柄群落的结构特征的三维图像来检查生物膜的发展。经常使用的工具是共聚焦激光扫描显微镜。我们提出了一个详细的协议,以生长,观察和分析呼吸道人类病原体,百日咳博德特氏菌的生物膜在空间和时间。

【背景】百日咳博德特氏菌(Bordetella pertussis)是上呼吸道的专性人类病原体,引起百日咳或百日咳(Mooi,2010; Dorji et al。,2018)。 B的生物膜。百日咳在各种人造表面上以及静态,摇动和流体流动条件下形成(Mishra et al。,2005; Sloan et al。,2007 ; Serra et al。,2011)。对这些生物膜的显微评估表明,这种细菌产生不规则形状的微集落,由流体通道分隔,嵌入由细胞外DNA(eDNA),蛋白质和多糖组成的外聚合物基质中(Parise et al。,2007; Sloan et al。,2007; Serra et al。,2008; ...

Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA
Author:
Date:
2018-06-20
[Abstract]  The mucosal surfaces of the gastrointestinal, respiratory, reproductive, and urinary tracts, and the surface of the eye harbor a resident microflora that lives in symbiosis with their host and forms a complex ecosystem. The protection of the vulnerable epithelium is primarily achieved by mucins that form a gel-like structure adherent to the apical cell surface. This mucus layer constitutes a physical and chemical barrier between the microbial flora and the underlying epithelium. Mucus is critical to the maintenance of a homeostatic relationship between the microbiota and its host. Subtle deviations from this dynamic interaction may result in major implications for health. The protocol in this article describes the procedures to grow low mucus-producing HT29 and high mucus-producing ... [摘要]  胃肠道,呼吸道,生殖道和泌尿道的粘膜表面以及眼睛表面都有一个居住的微生物群落,它们与宿主共生并形成一个复杂的生态系统。脆弱的上皮细胞的保护主要通过形成附着于顶端细胞表面的凝胶样结构的粘蛋白实现。该粘液层构成了微生物菌群和下层上皮之间的物理和化学屏障。粘液对维持微生物群与宿主之间的稳态关系至关重要。与这种动态互动的细微差异可能会对健康产生重大影响。本文中的方案描述了生长低粘液产生HT29和高粘液产生HT29-MTX-E12细胞的程序,维持细胞并通过ELISA将其用于粘液定量。此外,还介绍了如何评估分泌黏液的数量。该系统可用于研究粘液对抗细菌毒素的保护作用,例如测试不同培养条件对粘液产生的影响或分析分子通过粘液层的扩散。由于本方案中使用的ELISA可用于不同的物种和粘液蛋白,因此也可以使用其他细胞类型。

【背景】身体与外部环境的界面由粘膜表面形成。这些粘膜上皮组织可以在例如胃肠道,呼吸道,生殖道和尿道以及眼睛表面发现。由于它们暴露于外部环境中,许多微生物会聚集在这些组织中。因此,这些上皮细胞已经进化出多种防御机制来回应其易受微生物攻击的影响。许多防御性化合物被分泌到粘膜液中,包括粘蛋白,抗体,防御素,protegrin,聚集蛋白,cathelicidins,溶菌酶,组蛋白和一氧化氮(Kagnoff和Eckmann,1997,Lu等人,2002 ...

Small Molecule-Based Retinal Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Author:
Date:
2018-06-20
[Abstract]  Retinal degeneration leads to loss of light-sensing photoreceptors eventually resulting in vision impairment and impose a heavy burden on both patients and the society. Currently available treatment options are very limited and mainly palliative. Ever since the discovery of human pluripotent stem cell technologies, cell replacement therapy has become a promising therapeutic strategy for these patients and may help restore visual function. Reproducibly generating enriched retinal cells including retinal progenitors and differentiated retinal neurons such as photoreceptors using human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in a dish is an essential first step for developing stem cell-based therapies. In addition, this will provide a reliable and sufficient supply ... [摘要]  视网膜变性导致光感受器丧失,最终导致视力损害,并给患者和社会带来沉重的负担。目前可用的治疗方案非常有限,主要是姑息治疗。自从人类多能干细胞技术的发现以来,细胞替代疗法已成为这些患者的有希望的治疗策略,并可能有助于恢复视觉功能。使用人类胚胎干(ES)细胞和诱导多能干(iPS)细胞在培养皿中重现性地产生包括视网膜祖细胞和分化的视网膜神经元(例如光感受器)的富集视网膜细胞是开发基于干细胞的治疗的重要的第一步。此外,这将为研究疾病机制提供可靠和充足的人类视网膜细胞供应。在这里,我们描述了一种小分子视网膜诱导协议,已被用于生成视网膜祖细胞和分化的视网膜神经元,包括来自几个人ES和iPS细胞系的光感受器。通过该方案产生的视网膜细胞可以在视网膜下移植后的几个月内存活并且功能性地整合到正常和患病的小鼠视网膜中。

【背景】世界各地的一些团体正在开发用于从人多能干细胞产生特定细胞类型的方法。这些细胞可能在再生医学的未来作为替代细胞的来源中发挥关键作用。这些新产生的人类细胞在开发更好和更准确的人类疾病模型中非常有用,然后可用于发现具有更好功效和安全性的新药。

我们的工作重点是影响全球数百万人的视网膜退行性疾病,如黄斑变性和视网膜色素变性。视网膜中光感受器的死亡通常与这些疾病相关,并导致严重损伤或全部视力丧失。没有有效的药物治疗可以治愈这些疾病。
...

Comments