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Potato Dextrose Broth

土豆右旋糖肉汤
Company: Sigma-Aldrich
Catalog#: P6685
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Wheat Root-dip Inoculation with Fusarium graminearum and Assessment of Root Rot Disease Severity
Author:
Date:
2017-03-20
[Abstract]  Fusarium graminearum is one of the most common and potent fungal pathogens of wheat (Triticum aestivum) and other cereals, known for causing devastating yield losses and mycotoxin contaminations of food and feed. The pathogen is mainly considered as a paradigm for the floral disease Fusarium head blight, while its ability to colonize wheat plants via root infection has been examined recently. F. graminearum has a unique infection strategy which comprises complex, specialized structures and processes. Root colonisation negatively affects plant development and leads to systemic plant invasion by tissue-adapted fungal strategies. The pathosystem wheat root - F. graminearum makes available an array of research areas, such as (i) the relatively unknown root ... [摘要]  禾本科镰刀菌是小麦（小麦）和其他谷物中最常见和最有效的真菌病原体之一，已知会引起食品和饲料的破坏性产量损失和霉菌毒素污染。病原体主要被认为是镰刀菌枯萎病花粉病的典范，而最近已经检查了通过根系感染定植小麦植物的能力。 F。 graminearum 具有独特的感染策略，包括复杂的专门结构和过程。根系定殖对植物发育负面影响，并导致组织适应真菌策略的全身植物侵袭。小麦根系 - 小麦根。 graminearum 提供了一系列研究领域，例如（i）与坏死性病原体相对未知的根系相互作用; （ii）有助于（总体）镰刀菌抗性的基因和途径; （iii）诱导全身（全植株）抗性; （iv）各种宿主组织中的致病策略;和（v）对幼苗和成年植物（根/穗）感染的单基因型应答的年龄相关变化。提出的镰刀菌根腐病生物测定可以有效地感染小麦根，评估疾病的严重性和进展以及疾病动态的统计分析。

背景 根腐病（FRR）生物测定法使用根浸法进行接种，结合不同的疾病严重性参数测量。本协议主要适用于其他根真菌相互作用的研究。所提出的根浸接种被证明是一种有效和可靠的方法来研究小麦根 - 镰刀菌相互作用（表型和组织学）和筛选小麦基因型对根系感染的响应（Wang et al。 al 。，2015）。使用描述的方案，FRR疾病的遗传，分子和代谢组学方面同时经过不同研究方法的生物学重复和一致性观察结果的可靠结果进行了检查。这一点与在黄萎病的研究中所作的观察结果相对应，其特征在于在浸种或浸种方法方面的有效性和可靠性优于根浸渍（Trapero等人＆lt; ...

Trichoderma harzianum Root Colonization in Arabidopsis
Author:
Date:
2015-07-05
[Abstract]  Trichoderma is a soil-borne fungal genus that includes species with a significant impact on agriculture and industrial processes. In this article we show a detailed protocol of Trichoderma harzianum (T. harzianum) root invasion procedure described by Alonso-Ramírez et al. (2014). Some Trichoderma strains exert beneficial effects in plants through root colonization. They promote growth and development, modify root architecture, facilitate efficient nutrient use, or stimulate defenses against pathogens, although little is known about how this interaction takes place. For this purpose, Trichoderma-Arabidopsis hydroponic cultures were grown inside Phytatray II boxes, using mycelia obtained from spores of T. harzianum and Arabidopsis ... [摘要]  <木霉属是土壤传播的真菌属，包括对农业和工业过程具有显着影响的物种。 在本文中，我们展示了由alonso-ramírez等人（2014年）所描述的哈茨木霉的（哈茨木霉）根侵入程序的详细方案="" ）。="" 一些木霉菌株通过根定植在植物中发挥有益效果。="" 它们促进生长和发育，改变根结构，促进有效的营养物利用或刺激对病原体的防御，尽管关于如何发生这种相互作用了解甚少。="" 为此目的，使用从t孢子获得的菌丝体，在phytatray="" ii盒内培养木霉属拟南芥水培培养物。="" harzianum="" 和拟南芥（拟南芥）幼苗。="">

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