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Author:
Date:
2015-05-20
[Abstract] NLRP3 inflammasome is a multiprotein complex responsible for the activation of inflammatory caspase-1, resulting in processing and release of pro-inflammatory cytoquines IL-1β and IL-18 (Schroder and Tschopp, 2010). This inflammasome is composed of the sensor protein NLRP3 connected to caspase-1 through the adaptor protein ASC (apoptosis-associated speck-like protein with a caspase-recruitment domain) (Schroder and Tschopp, 2010). We and others have reported that upon inflammasome activation functional oligomeric inflammasome particles of NLRP3 and ASC were released from cells, acting as danger signals to amplify inflammation by promoting the activation of caspase-1 extracellularly (Baroja-Mazo et al., 2014; Franklin et al., 2014).
Studying the extracellular ...
[摘要] NLRP3炎症小体是负责炎症半胱天冬酶-1活化的多蛋白复合物，导致促炎细胞因子IL-1β和IL-18的加工和释放（Schroder和Tschopp，2010）。这种炎症小体由通过衔接蛋白ASC（凋亡相关的斑点样蛋白与半胱氨酸蛋白酶募集结构域）连接到caspase-1的传感蛋白NLRP3组成（Schroder和Tschopp，2010）。我们和其他人已经报道，在炎症小体激活时，NLRP3和ASC的功能性寡聚炎症小体颗粒从细胞中释放，充当危险信号，通过促进细胞外caspase-1的活化来扩增炎症（Baroja-Mazo等，
通过纯化ASC的重组颗粒或组成型激活的NLRP3突变体来研究寡聚ASC和NLRP3炎症小体颗粒的细胞外功能是可能的与相关的cryopyrin相关周期性综合征（CAPS，突变p.D303N），都标记有黄色荧光蛋白（YFP），并在HEK293细胞中表达。通过重组ASC或突变体NLRP3在HEK293细胞中的表达导致它们自发聚集成斑点（Baroja-Mazo等人，2014）的事实促进了纯化过程，并且方案最初从Fernandes-Alnemri和Alnemri（2008年）。
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