Triton X-100

Company: NACALAI TESQUE

Catalog#: 35501-15

Purification of Native Dentilisin Complex from Treponema denticola by Preparative Continuous Polyacrylamide Gel Electrophoresis and Functional Analysis by Gelatin Zymography

Pachiyappan Kamarajan, John C. Timm, M. Paula Goetting-Minesky, Erin T. Malone, Sean Ganther, Allan Radaic, Christian Tafolla, J. Christopher Fenno and Yvonne L. Kapila

Published online: 2024-04-05

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Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies

Author:

Tomoko Kubori and Hiroki Nagai,

Date:

2017-04-20

[Abstract] Legionella possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from L. pneumophila aiming to perform biochemical and transmission electron microscopy analyses. [摘要] 军团菌具有关键的分泌机制，以将毒力因子递送至真核宿主细胞。在本协议中，我们描述了从L / L分离Dot / Icm IV型分泌系统的天然核心复合物的步骤。肺炎支原体旨在进行生物化学和透射电子显微镜分析。

嗜肺军团菌是革兰氏阴性细菌病原体，其导致被称为退伍军人病的肺部感染（Fields等人，2002）。 L。嗜肺杆菌利用由 dot / cm 基因编码的IV型分泌系统（T4SS）将大约300种细菌蛋白转运到其真核宿主细胞质中以劫持细胞过程（Hubber和Roy， 2010）。由超过20种蛋白组成，T4SS是建立在细菌内膜和外膜上的纳米机器（Nagai和Kubori 2011; Kubori和Nagai 2016）。 Dot / Icm T4SS的核心复合物是系统的生物化学稳定部分，并形成桥接内膜和外膜的输送导管（Kubori等人，2014）。核心复合物由至少五种蛋白质组成;三种外膜相关蛋白，DotC，DotD和DotH，以及两种内膜蛋白DotF和DotG（Vincent等人，2006）。基于来自鼠伤寒沙门氏菌的另一种细菌纳米机械的III型分泌系统的生物化学分离方法（Kubori等人，1998; Marlovits等人， ...

ELISA Detection of Endogenous Serum Albumin in the Mouse Brain: A Measure of Extravasation Following Brain Injury

Author:

Shotaro Michinaga and Yutaka Koyama,

Date:

2015-05-05

[Abstract] After stroke and brain contusion, serum proteins extravasate into nerve tissue through disrupted blood-brain barrier (BBB). Because extravasations of serum proteins result in vasogenic brain edema, serum albumin level in the brain is an indicator of BBB disruption and brain edema after brain insults. In this protocol, extravasation of endogenous albumin is measured in the damaged mouse brain, which would be valuable in the evaluation of vasogenic brain edema formation (Michinaga et al., 2014). [摘要] 在中风和脑挫伤后，血清蛋白通过破裂的血脑屏障（BBB）外渗到神经组织中。因为血清蛋白的外渗导致血管性脑水肿，脑中的血清白蛋白水平是脑损伤后BBB破坏和脑水肿的指标。在该方案中，在损伤的小鼠脑中测量内源性白蛋白的外渗，这在评价血管性脑水肿形成中是有价值的（Michinaga等人，2014）。

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