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Company: Denville Scientific
Catalog#: P3193-S
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Immunoprecipitation of Proteins in Caenorhabditis elegans
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Date:
2015-04-05
[Abstract]  Immunoprecipitation (IP) is a biochemical technique to precipitate a protein out of solution using an antigen that can specifically bind to that protein. IP can be performed to isolate and concentrate one particular protein from a sample of thousands of different proteins. IP is also readily performed to pull down interacting proteins of complexes out of solution. This protocol outlines the methods used to IP proteins in whole worm lysates and their preparation for detection on Western blots using denaturing conditions. [摘要]  免疫沉淀(IP)是使用可以特异性结合该蛋白质的抗原将蛋白质从溶液中沉淀出来的生物化学技术。 IP可以从数千种不同蛋白质的样品中分离和浓缩一种特定蛋白质。 IP也容易进行以从溶液中取出复合物的相互作用蛋白。 该协议概述了用于IP蛋白在整个蠕虫裂解物中的方法及其用于使用变性条件在蛋白质印迹上检测的方法。

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