{{'Search' | translate}}
 

Ethidium Bromide extrapure

溴化乙锭外

Company: SRL Sisco Research Laboratories
Catalog#: 17220
Bio-protocol()
Company-protocol()
Other protocol()

Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Author:
Date:
2015-03-05
[Abstract]  Small RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs) and other noncoding RNAs including snRNA and snoRNA have tremendous impact on eukaryotic gene regulation. Extraction of high quality small RNAs is an important prerequisite for experimental analyses of miRNAs. This will prevent RNA degradation and remove associated contaminations including polyphenols, polysaccharides and other secondary metabolites. In this protocol we describe a simple way to isolate small RNAs from the leaf tissues of Vigna mungo combining the protocols of two commercially available kits with some modifications. [摘要]  小RNA如微小RNA(miRNA),小干扰RNA(siRNA)和其他非编码RNA(包括snRNA和snoRNA)对真核基因调控具有巨大的影响。 高质量小RNA的提取是miRNA的实验分析的重要先决条件。 这将防止RNA降解和去除相关的污染物,包括多酚,多糖和其他次生代谢物。 在这个协议中,我们描述了一种简单的方法,从猕猴桃的叶组织中分离小RNAs,结合两个商业上可用的试剂盒的协议与一些修改。

Comments