Characterization of HBV Isolates from Patient Serum Samples and Cloning
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Author:
Date:
2015-12-20
[Abstract] Hepatitis B virus (HBV) mutants can lead to vaccine failure, diagnostic failure of HBV detection, increase viral replication and resistance to antiviral agents. To study the biological characteristics of these mutations may contribute to our knowledge on viral pathogenesis. Therefore, it is essential to isolate and characterize HBV strains from patients. Here we describe the experimental methods to isolate and clone HBV DNA from patient serum. The method will facilitate isolation and functional analysis of new HBV variants.
[摘要] 乙型肝炎病毒(HBV)突变体可导致疫苗失败,HBV检测的诊断失败,增加病毒复制和对抗病毒剂的抗性。 研究这些突变的生物学特性可能有助于我们对病毒发病机制的了解。 因此,必须从患者中分离和表征HBV病毒株。 在这里我们描述从患者血清中分离和克隆HBV DNA的实验方法。 该方法将促进新的HBV变体的分离和功能分析。
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RNA Editing Detection by Direct Sequencing
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Author:
Date:
2015-03-05
[Abstract] RNA editing is a widespread post-transcriptional phenomenon through which primary RNA sequences are altered by nucleotide insertion/deletion or base conversion. It occurs in a variety of organisms and cooperates with alternative splicing in increasing both proteomic and transcriptomic complexity. We describe here a method allowing RNA editing events detection by performing direct sequencing of both genomic DNA and cDNA from the same source.
[摘要] RNA编辑是广泛的转录后现象,通过其通过核苷酸插入/缺失或碱基转化来改变初级RNA序列。 它发生在多种生物体中,并与增加蛋白质组和转录组复杂性的可变剪接协作。 我们在这里描述一种允许通过对来自相同来源的基因组DNA和cDNA进行直接测序来进行RNA编辑事件检测的方法。
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