| Sample Preparation for Correlative Light and Electron Microscopy (CLEM) Analyses in Cellular Microbiology
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Author:
Date:
2015-10-05
[Abstract] Dynamic processes in cells are usually monitored by live cell fluorescence microscopy. Unfortunately, this method lacks the ultrastructural information about the structure of interest (SOI). Currently, electron microscopy (EM) is the best tool to achieve highest spatial resolution. In addition, correlative light and electron microscopy (CLEM) analysis of the same structure allows combining authentic live cell imaging with the resolution power of EM. Additionally the reference space of the SOI is revealed. Our CLEM analyses of HeLa cells allow tracing the morphology and dynamic behavior of intracellular micro-compartments in living cells and their ultrastructure and subcellular organization in a highly resolved manner.
[摘要] 通常通过活细胞荧光显微镜监测细胞中的动态过程。 不幸的是，这种方法缺乏关于感兴趣的结构（SOI）的超结构信息。 目前，电子显微镜（EM）是实现最高空间分辨率的最佳工具。 此外，相同结构的相关光和电子显微镜（CLEM）分析允许组合真实活细胞成像与EM的分辨率功率。 此外，揭示了SOI的参考空间。 我们对HeLa细胞的CLEM分析允许以高度解析的方式追踪活细胞中的细胞内微隔室及其超微结构和亚细胞组织的形态和动态行为。
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| In vitro Dynamic Model of a Catheterized Bladder and Biofilm Assay
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Author:
Date:
2015-01-20
[Abstract] Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI) which represent the most frequent nosocomial infections. Understanding of factors relevant for CAUTI pathogenesis and evaluation of new therapeutics or interference strategies requires a model system that mirrors the physico-chemical conditions prevailing in a catheterized human bladder. The described in vitro dynamic model of a catheterized bladder enables to emulate many of the characteristics of a catheterized human bladder albeit in the absence of a bladder epithelium. A minor modification compared to the original model system (Stickler, et al., 1999) allows temperature maintenance of the top 10 cm of the catheter, thereby enabling ...
[摘要] 导管上的生物膜形成被认为有助于导管相关尿路感染（CAUTI）的持久性，其代表最常见的医院感染。 理解与CAUTI发病机理相关的因素和评价新的治疗或干扰策略需要一个反映导尿人膀胱中存在的物理化学条件的模型系统。 所描述的导管插入的膀胱的体外动态模型使得能够模拟导管插入的人膀胱的许多特征，尽管在没有膀胱上皮的情况下。 与原始模型系统（Stickler，et al。，1999）相比较小的修改允许导管的顶部10cm的温度维持，从而能够可再生地监测内部导管表面上的生物膜形成。
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