{{'Search' | translate}}

BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

BCECF,AM(2'',7''-双 - (2-羧乙基)-5-(和-6) - 羧基荧光素,乙酰氧基甲基酯)

Company: Thermo Fisher Scientific
Catalog#: B1170
Other protocol()

A Protocol for Measurement of Intracellular pH
[Abstract]  Intracellular pH (pHi) is an important physiological determinant of enzyme activity and cellular function (Kurkdjian and Guern, 1989). All proteins depend on a tightly regulated pH to maintain their structure and function. Protonation–deprotonation events can dictate the charge of biological surfaces and are integral steps in many metabolic reactions (Casey et al., 2010). Moreover, the proton gradient across the mitochondrial membrane is used to generate cellular energy and support other mitochondrial processes. As a result, cells have developed multiple mechanisms to maintain a narrow range of pHi in response to extra- and intracellular fluctuations in pH (Orij et al., 2012). Here, we describe a protocol for pHi measurement in live cells ... [摘要]  细胞内pH(pH )是酶活性和细胞功能的重要生理决定因素(Kurkdjian和Guern,1989)。所有蛋白质依赖于严格调节的pH以维持其结构和功能。质子化 - 去质子化事件可以决定生物表面的电荷,并且是许多代谢反应中不可或缺的步骤(Casey等人,2010)。此外,穿过线粒体膜的质子梯度用于产生细胞能量和支持其他线粒体过程。因此,细胞已经发展了多种机制以响应于pH的胞外和细胞内波动维持pH范围的窄范围(Orij等人,2012)。在这里,我们描述了在使用荧光显微镜和pH敏感染料2',7'-双 - (2-羧乙基)-5-(和-6-氨基己酸)的活细胞中pH pH测量的方案, ) - 羧基荧光素乙酰氧基甲基酯(BCECF-AM)。该方法最近用于测定细胞内pH变化对全局组蛋白乙酰化水平的影响(McBrian等人,2013)。

Fluorescent Dye Based Measurement of Vacuolar pH and K+
[Abstract]  Availability of ion specific fluorescent dyes has enabled the possibility to perform in vivo ion specific measurements using live cell imaging in many cellular compartments (Krebs et al., 2010; Bassil et al., 2011; Halperin and Lynch, 2003; Swanson et al., 2011; O'Connor and Silver, 2007). The importance of ion and pH homeostasis of intracellular compartments, including the vacuole, to cell growth is critical and well established (Krebs et al., 2010; Bassil et al., 2011).
* Dedicated to Stephen Halperin who tragically and unexpectedly passed away.
[摘要]  离子特异性荧光染料的可用性使得能够在许多细胞区室中使用活细胞成像在体内进行离子特异性测量(Krebs等人,2010; Bassil 2011; Halperin和Lynch,2003; Swanson等人,2011; O'Connor和Silver,2007)。 细胞内区室(包括液泡)的离子和pH稳态对细胞生长的重要性是关键的并且得到充分确立(Krebs等人,2010; Bassil等人, 2011)。