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Company: Capitol Scientific

Catalog#: 357572

Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae

Gang Li, Margarita Marroquin-Guzman and Richard A. Wilson

Published online: 2015-11-05

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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans

Author:

Germano Cecere and Alla Grishok,

Date:

2014-12-20

[Abstract] The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope. [摘要] RNA染色质免疫沉淀测定（RNA-ChIP）允许使用体内与甲醛交联，随后RNA-蛋白复合物的免疫沉淀来检测和定量RNA-蛋白质相互作用。在这里我们描述我们已经适应于秀丽隐杆线虫（ C. elegans ）的RNA-ChIP协议以检测核Argonaute CSR-1（染色体分离和RNAi缺陷）蛋白及其目标新生RNA。我们使用表达与N-末端3x FLAG表位融合的CSR-1蛋白的重组长同种型的转基因菌株。

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7mL Dounce Tissue Grinder Set with Stainless Steel

7mL Dounce组织研磨机用不锈钢

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