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Company: Thermo Fisher Scientific

Catalog#: 85111

Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae

Gang Li, Margarita Marroquin-Guzman and Richard A. Wilson

Published online: 2015-11-05

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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans

Author:

Germano Cecere and Alla Grishok,

Date:

2014-12-20

[Abstract] The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope. [摘要] RNA染色质免疫沉淀测定（RNA-ChIP）允许使用体内与甲醛交联，随后RNA-蛋白复合物的免疫沉淀来检测和定量RNA-蛋白质相互作用。在这里我们描述我们已经适应于秀丽隐杆线虫（ C. elegans ）的RNA-ChIP协议以检测核Argonaute CSR-1（染色体分离和RNAi缺陷）蛋白及其目标新生RNA。我们使用表达与N-末端3x FLAG表位融合的CSR-1蛋白的重组长同种型的转基因菌株。

Proteolytic Fragment Isolation and Analysis (ex. N-terminal GST-tagged CLAVATA3 Protein GST-CLV3)

Author:

Jun Ni,

Date:

2012-07-20

[Abstract] It has become clear that the post-embryonic growth and development of plants requires properly controlled short distance cell-to-cell communication not only through the historically well-known phytohormones, but also through secreted small peptide signals. This protocol demonstrates an example of how to isolate small peptides (< 10 daltons) from complex protein mixtures (e.g. cauliflower meristem protein extraction) for MS/MS analysis. [摘要] 已经变得清楚的是，植物的后胚胎生长和发育需要适当控制的短距离细胞与细胞的通信，不仅通过历史上熟知的植物激素，而且通过分泌的小肽信号。该方案展示了如何从复杂蛋白质混合物（例如花椰菜分生组织蛋白质提取）中分离小肽（<10道尔顿）用于ms/ms分析的实例。 s分析的实例。="">

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Triton^TM X-100 Surfact-Amps^TM Detergent Solution

Triton ^TM X-100 Surfact-Amps 洗涤剂溶液