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Neurobasal® Medium, minus phenol red

Neurobasal ®培养基,不含酚红

Company: Thermo Fisher Scientific
Catalog#: 12348017
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Generation of Neuron-enriched Cultures (Method 2)
Author:
Date:
2011-11-05
[Abstract]  Neuron-enriched cultures are a useful tool to study neuronal development and the molecular pathways that come in to play during neuronal death in various neurological disorders. This protocol is for generating midbrain neuronal cultures from late embryo rodent brain. This method uses Neurobasal medium and B27 serum-free supplement. The long-lasting (> 4 weeks) midbrain neuron-enriched cultures generated following this protocol have been wildly used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder. The neuron-enriched cultures prepared following this protocol will contain < 10% astroglia. the usage of b27 serum-free supplement will definitively increase the cost. this protocol has been developed and improved over the years by various researchers in dr. hong’s lab, especially dr. bin liu. 10%="" astroglia.="" the="" usage="" of="" b27="" serum-free="" supplement="" will="" definitively="" increase="" the="" cost.="" this="" protocol="" has="" been="" developed="" and="" improved="" over="" the="" years="" by="" various="" researchers="" in="" dr.="" hong’s="" lab,="" especially="" dr.="" bin=""> ... [摘要]  神经元富集的培养物是研究神经元发育和在神经元死亡期间在各种神经疾病中发挥的分子途径的有用工具。 该协议是用于从晚期胚胎啮齿动物脑产生中脑神经元培养物。 该方法使用Neurobasal培养基和B27无血清补充剂。 根据该方案产生的持续(> 4周)中脑神经元富集培养物已被广泛用于研究帕金森病的发病机理,帕金森病是最常见的神经退行性运动障碍。 按照该方案制备的富含神经元的培养物将含有<10%星形胶质细胞。 使用b27无血清补充剂将明显增加成本。="">

Generation of Neuron-enriched Cultures (Method 1)
Author:
Date:
2011-11-05
[Abstract]  This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This method uses cytosine β-D-arabinofuranoside in the cultures to suppress the proliferation of glial cells. Seven days after the seeding, the neuron-enriched cultures prepared following this protocol will contain less than 10% glia cultures. The concentrations of β-D-arabinofuranoside should be adjusted according to your culture condition. This protocol has been developed and improved over the years by various researchers in Dr. Hong’s lab, especially Dr. Bin Liu. [摘要]  这个协议将指导您通过从晚胚胎小鼠大脑产生中脑神经元文化的过程。 这些文化作为一个有用的工具,研究神经元死亡期间各种神经系统疾病的分子途径。 该方法在培养物中使用胞嘧啶β-D-阿拉伯呋喃糖苷抑制神经胶质细胞的增殖。 接种后7天,按照该方案制备的富含神经元的培养物将含有少于10%的胶质细胞培养物。 应根据您的培养条件调整β-D-阿拉伯呋喃糖苷的浓度。 这个协议已经由Hong博士实验室的各种研究人员,尤其是刘博士多年来开发和改进。

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