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Microcentrifuge Tubes with Tether Cap

带有系绳盖的微量离心管

Company: Thermo Fisher Scientific
Catalog#: 02-707-355
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Cyclohexane Diamine Tetraacetic Acid (CDTA) Extraction of Plant Cell Wall Pectin
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Date:
2014-12-20
[Abstract]  The goal of this procedure is to extract pectin from plant cell walls. Pectins are galacturonic acid containing polymeric sugars that are important components of plant cell walls. Various procedures aimed at studying plant cell wall components require the extraction of pectin. Pectin is synthesized in the Golgi apparatus in a highly esterified fashion and is de-esterified in the cell wall (Mohnen, 2008). Pectin is generally water soluble. De-esterified pectin can form so-called “egg-box structures” in the presence of Ca2+ ions (Mohnen, 2008; Harholt et al., 2010). Pectin in these “egg-box structures” is cross-linked and less soluble. Cyclohexane diamine tetraacetic acid (CDTA) chelates Ca2+ ions and hence allows extraction of Ca2+ cross-linked ... [摘要]  该程序的目的是从植物细胞壁提取果胶。 果胶是含有聚合糖的半乳糖醛酸,其是植物细胞壁的重要组分。 旨在研究植物细胞壁组分的各种方法需要提取果胶。 果胶以高度酯化的方式在高尔基体中合成,并在细胞壁中脱酯(Mohnen,2008)。 果胶通常是水溶性的。 脱酯的果胶在Ca 2+ 2+离子存在下可形成所谓的"蛋盒结构"(Mohnen,2008; Harholt等人,2010)。 这些"蛋盒结构"中的果胶是交联的并且溶解性较差。 环己烷二胺四乙酸(CDTA)螯合Ca 2+ 2+离子,因此允许从细胞壁提取Ca 2+ 2+交联的果胶。

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