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Glutathione

谷胱甘肽
Company: Thermo Fisher Scientific
Catalog#: 78259
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Proteolytic Fragment Isolation and Analysis (ex. N-terminal GST-tagged CLAVATA3 Protein GST-CLV3)
Author:
Date:
2012-07-20
[Abstract]  It has become clear that the post-embryonic growth and development of plants requires properly controlled short distance cell-to-cell communication not only through the historically well-known phytohormones, but also through secreted small peptide signals. This protocol demonstrates an example of how to isolate small peptides (< 10 daltons) from complex protein mixtures (e.g. cauliflower meristem protein extraction) for MS/MS analysis. [摘要]  已经变得清楚的是，植物的后胚胎生长和发育需要适当控制的短距离细胞与细胞的通信，不仅通过历史上熟知的植物激素，而且通过分泌的小肽信号。 该方案展示了如何从复杂蛋白质混合物（例如花椰菜分生组织蛋白质提取）中分离小肽（<10道尔顿）用于ms/ms分析的实例。 s分析的实例。="">

Expression and Purification of GST-tagged Proteins from E. coli
Author:
Date:
2011-09-20
[Abstract]  This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended. [摘要]  该协议描述了用于小规模和大规模表达和纯化GST蛋白的方法。 由于蛋白质的多样性，总是推荐小规模的表达和纯化试验。

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