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DL-2-Amino-5-phosphonopentanoic acid

DL-2-氨基-5-膦酰基戊酸

Company: Sigma-Aldrich
Catalog#: A5282
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Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons
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2014-12-05
[Abstract]  This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons. [摘要]  该协议包括使用探针SynaptopHluorin的荧光测量的荧光测量的整个过程,所述探针是pH依赖性GFP变体,其荧光在囊泡释放时由于酸性囊泡中的荧光猝灭而在突触上增加。 这种技术提供了一种遗传工具来监测突触小泡实时回收培养的海马神经元。

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