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Peptone from animal tissue

来自动物组织的蛋白胨

Company: Sigma-Aldrich
Catalog#: P5905
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Pathogenicity Assay of Verticillium nonalfalfae on Hop Plants
Author:
Date:
2017-03-20
[Abstract]  Verticillium nonalfalfae is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant’s xylem and causes wilt disease symptoms by secreting different virulence factors. Hop (Humulus lupulus) is a primary host of V. nonalfalfae, so it is used as a model plant for studying this phytopathogenic fungus. Artificial infections of hop plants and disease scoring are prerequisites for studying the pathogen’s virulence/pathogenicity and its interaction with hop plants. In this protocol, we describe the root dipping inoculation method for conducting pathogenicity assay of V. nonalfalfae on hop plants. [摘要]  苜蓿轮枝菌是一种土壤传播的植物病原体,通过根感染其宿主。它传播在植物的木质部,并通过分泌不同的毒力因子引起枯萎病症状。 Hop(umulus lupulus)是V主要的主机。非苜蓿,因此它被用作研究这种植物病原真菌的示范植物。啤酒植物的人工感染和疾病评分是研究病原体的毒力/致病性及其与啤酒花植物的相互作用的先决条件。在该方案中,我们描述了用于进行V的致病性测定的根浸渍接种方法。非苜蓿植物。

背景 轮枝孢属 spp。感染400多种不同的宿主植物,每个物种都有自己的宿主范围。主要的主持人。非苜蓿是跳。然而,跳跃具有用作致病性测定的测试植物的几个缺点;例如,它是一种多年生植物,需要经历休眠阶段。因此,从春季到夏末,植物只能用于几年的致病性测定。 Hop品种在温室中作为软木切片繁殖繁殖,或作为从砧木的休眠扦插。种子是通过交叉雌性和雄性植物获得的,并且仅用于育种目的。根浸渍接种方法已被广泛应用于轮枝孢属菌株的致病性测定。在其他植物宿主上,例如,番茄(Fradin等人,2009),N。 (Klosterman等人,2011)和拟南芥拟南芥(Ellendorff等人,2009)。

Xylem Sap Extraction Method from Hop Plants
Author:
Date:
2017-03-20
[Abstract]  Verticillium wilt is one of the most important diseases on hop that significantly influence continuation of production on affected areas. It is caused by the soil borne vascular pathogen Verticillium nonalfalfae, which infects plants through the roots and then advances through the vascular (xylem) system. During infection, V. nonalfalfae secretes many different virulence factors. Xylem sap of infected plants is therefore a rich source for investigating the molecules that are involved in molecular interactions of Verticillium – hop plants. This protocol provides instructions on how to infect hop plants with V. nonalfalfae artificially and how to obtain xylem sap from hop plants. [摘要]  黄萎病是最重要的疾病之一,对受影响地区的生产继续产生重大影响。它是由土壤传播的血管病原体疣黄萎病引起的,其通过根感染植物,然后通过血管(木质部)系统前进。感染期间,非苜蓿科分泌许多不同的毒力因子。因此感染植物的木质部汁液因此是调查涉及轮枝孢属植物的分子相互作用的分子的丰富来源。该协议提供有关如何用V感染啤酒花植物的说明。人工制备非苜蓿,如何从啤酒花植物获得木质部汁液。

背景 从植物中提取木质部汁液主要用于木质部汁液蛋白质组学的研究,并且已经使用各种方法从植物木质部组织中提取汁液。 Buhtz等人。 (2004)使用手持式移液器从切割的植物茎收集木质部汁液,用于比较不同植物(西兰花,油菜,南瓜和黄瓜)中的木质部蛋白质组。采用相同的方法从欧洲油菜中收集木质部汁液(Kehr等,2005),芸苔属(Ligat et al。,et al。 al。,2011)和大豆(Subramanian等人,2009)。 Alvarez等人。 (2006)使用“根压力”提取玉米木质部汁液蛋白质组,如Goodger等人所述。 (2005)。 Dafoe和Constabel(2009)使用安装在木材上的Tygon管,从混合杨树收集木质部汁液,并且不施加额外的压力。关于木质部汁液的蛋白质含量的信息也适用于苹果,梨和桃子(Biles and ...

Establishment of a Symbiotic in vitro System between a Green Meadow Orchid and a Rhizoctonia-like Fungus
Author:
Date:
2015-05-20
[Abstract]  Symbiotic orchid seed germination in an in vitro system allows the growth of mycorrhizal protocorms and plantlets for scientific purposes. Orchids in nature need to establish a mycorrhizal symbiosis with fungal partners to germinate and develop into adult plants. Here we present a protocol for symbiotic germination of the terrestrial Mediterranean green meadow orchid Serapias vomeracea. The fungal symbiont Tulasnella calospora (T. calospora) (Basidiomycetes, Cantharellales) was chosen because of its common occurrence (Girlanda et al., 2011), its ability to grow in culture and compatibility in germination assays. T. calospora is one of the most common rhizoctonia-like fungi associated with terrestrial as well as epiphytic orchids. [摘要]  在体外系统中的共生兰花种子萌发允许用于科学目的的菌根原球茎和小植物的生长。 兰花本质上需要建立与真菌合作者的菌根共生,以发芽和发育成成虫植物。 在这里我们提出了地中海地中海绿色草地兰花 Serapias vomeracea的共生萌发的协议。 选择真菌共生菌(Talsnella calospora)( T。calospora )(担子菌门,Cantharellales),因为其常见的发生(Girlanda等人,2011 ),其在培养中生长的能力和在发芽测定中的相容性。 T。 calospora 是与陆生以及附生兰花相关的最常见的丝孢菌样真菌之一。

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