Poly-D-lysine hydrobromide
Company: Sigma-Aldrich
Catalog#: P7280
Product evaluation:
Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
Author:
Date:
2017-11-05
[Abstract]  There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation in a reductionist model. While the neurosphere assay is primarily performed to directly study the proliferative and differentiation potential of cells ...

Neurosphere Co-culture Assay
Author:
Date:
2016-08-05
[Abstract]  The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, we established a co-culture system. We investigate the formation of neurosphere under different co-culture conditions by using primary niche cells. ...

Midbrain Neuron-glia Mixed Cultures
Author:
Date:
2012-04-05
[Abstract]  Mixed neuron-glia cultures provide a unique tool to study cellular contribution and molecular pathways in various neurological disorders. They are also invaluable for exploring neuron-glia interaction under physiological and pathological conditions. The relatively long-lasting midbrain neuron-glia mixed cultures generated following this protocol have been widely used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder.

Comments
  • Bio-protocol’s mission is to improve research reproducibility. Please join our effort by sharing your experience on the reagents/equipment that you have used.
Similar products