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Hydrochloric Acid

盐酸

Company: Thermo Fisher Scientific
Catalog#: A144S-500
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Activity Assays for Bacteriophage Endolysin PlyPy
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Date:
2014-09-20
[Abstract]  Bacterial viruses (bacteriophages) escape and kill their host by degrading the bacterial peptidoglycan layer through the mechanism of enzymes called endolysins: peptidoglycan degrading enzymes. The method included here is useful for the initial characterization of any endolysin, regardless of the specific catalytic domain (as long as the activity results in a reduction in the optical density), in order to determine its optimal enzymatic (lytic) activity. This protocol is specific for the Streptococcus pyogenes phage endolysin PlyPy, but can be adapted for any peptidoglycan degrading enzyme. [摘要]  细菌病毒(噬菌体)通过称为内溶素:肽聚糖降解酶的酶的机制降解细菌肽聚糖层而逃脱并杀死它们的宿主。 本文包括的方法可用于任何内溶素的初始表征,而不管具体的催化结构域(只要活性导致光密度降低),以确定其最佳酶(裂解)活性。 该方案对于化脓链球菌噬菌体内溶素PlyPy是特异性的,但可以适用于任何肽聚糖降解酶。

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