SYPRO® Orange Protein Gel Stain
Company: Thermo Fisher Scientific
Catalog#: S6651
Product evaluation:
In vitro Chitin Binding Assay
[Abstract]  Chitin is polymer of N-acetylglucosamine (GlcNAc) found in the exoskeleton of arthropods and the fungal cell wall. GlcNAc is also implicated in bacterial development, adherence, and signal transduction but can also be used as a carbon source. In vitro chitin binding assay is performed to determine the affinity of a purified protein to the chitin molecule. The principle is based on the co-sedimentation of chitin-binding proteins together with chitin-coated beads.

Fluorometric Estimation of Viral Thermal Stability
[Abstract]  Differential Scanning Fluorimetry (DSF) is a rapid, economical, and a straightforward technique for estimating the thermal stability of proteins. The principle involves the binding of a fluorescent dye to thermally exposed hydrophobic pockets of a protein. The dyes used in this technique are highly fluorescent in a non-polar environment and are quenched when exposed to aqueous solution. The change in fluorescence can be used to follow unfolding of proteins induced by temperature, pH, or ...

  • Bio-protocol’s mission is to improve research reproducibility. Please join our effort by sharing your experience on the reagents/equipment that you have used.
Similar products