| Ear Inflammation and Whole-mount Ear Staining
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Author:
Date:
2016-10-20
[Abstract] The recruitment of circulating neutrophils from the bloodstream to the site of inflammation represents one of the earliest events during an innate immune response. During this response, neutrophils tether and roll along the vessel walls before transmigrating across the endothelium into the interstitial space to exert their functions. Here, we describe a protocol for the staining of intravascular and tissue-localized neutrophils following contact sensitization of the skin with croton oil. Visualization of the neutrophilic distribution in skin provides for a better interpretation of the local immune response.
[摘要] 循环嗜中性粒细胞从血流中募集到炎症部位代表先天免疫反应期间的最早期事件之一。在这种反应期间，嗜中性粒细胞在通过内皮迁移到间质空间以发挥其功能之前沿血管壁束缚和滚动。在这里，我们描述染色的血管内和组织定位中性粒细胞皮肤与巴豆油接触致敏后的协议。皮肤中嗜中性粒细胞分布的可视化提供了局部免疫应答的更好解释。
[背景] 炎症后中性粒细胞分布在皮肤中的表征代表了理解其专门功能的重要途径。即使通过流式细胞术广泛表征嗜中性粒细胞在炎症皮肤中的募集（Hampton等人，2015; Stock em 。，2014），这种技术提供有限的洞察中性粒细胞的血管内与间质定位。再循环和组织定位中性粒细胞展示不同的表型和功能，这使得他们的歧视需要识别当地免疫反应的关键参与者。随着活体显微镜（IVM）的发展，使得荧光标记的免疫细胞在体内的直接可视化成为可能。然而，由于IVM的高成本，这种强大的成像工具对研究人员的可访问性是有限的。在这里，我们提出了高分辨率静电成像的中性粒细胞在皮肤中使用成本效益试剂和通常可用的共聚焦激光扫描显微镜的替代协议。
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| Luminescence-based Antiviral Assay for Hepatitis E Virus
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Author:
Date:
2014-08-05
[Abstract] Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate potential new inhibitors of HEV replication, a luminescence-based replicon assay is particularly useful since it offers a rapid read-out and does not pose any biosafety risks (Debing et al., 2014).
[摘要] 戊型肝炎病毒（HEV）是全世界急性肝炎的主要原因之一。 感染在孕妇中特别严重，已知慢性戊型肝炎发生在免疫受损的患者中。 目前的治疗（利巴韦林或聚乙二醇化α干扰素）具有严重的副作用，不能用于所有患者。 为了评价潜在的新的HEV复制抑制剂，基于发光的复制子测定法是特别有用的，因为其提供了快速读出，并且不会引起任何生物安全风险（Debing等人，2014） 。
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