| Infectious Virus Yield Assay for Hepatitis E Virus
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Author:
Date:
2014-08-05
[Abstract] Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate potential new inhibitors of HEV replication, a virus yield assay can be employed in which the amount of viral RNA progeny released into the culture medium is quantified by reverse-transcription quantitative PCR (RT-qPCR) (Debing et al., 2014).
[摘要] 戊型肝炎病毒（HEV）是全世界急性肝炎的主要原因之一。 感染在孕妇中特别严重，已知慢性戊型肝炎发生在免疫受损的患者中。 目前的治疗（利巴韦林或聚乙二醇化α干扰素）具有严重的副作用，不能用于所有患者。 为了评估潜在的新的HEV复制抑制剂，可以使用病毒产量测定法，其中释放到培养基中的病毒RNA子代的量通过逆转录定量PCR（RT-qPCR）（Debing等人， al 。，2014）。
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| Luminescence-based Antiviral Assay for Hepatitis E Virus
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Author:
Date:
2014-08-05
[Abstract] Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate potential new inhibitors of HEV replication, a luminescence-based replicon assay is particularly useful since it offers a rapid read-out and does not pose any biosafety risks (Debing et al., 2014).
[摘要] 戊型肝炎病毒（HEV）是全世界急性肝炎的主要原因之一。 感染在孕妇中特别严重，已知慢性戊型肝炎发生在免疫受损的患者中。 目前的治疗（利巴韦林或聚乙二醇化α干扰素）具有严重的副作用，不能用于所有患者。 为了评价潜在的新的HEV复制抑制剂，基于发光的复制子测定法是特别有用的，因为其提供了快速读出，并且不会引起任何生物安全风险（Debing等人，2014） 。
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| Protocol for T-cell Adhesion Strength on Tumor Cells under Flow Conditions
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Author:
Date:
2013-10-20
[Abstract] This method allows evaluating the relative adhesion strength between T lymphocytes and specific adherent target cells using a shear force in flow chambers. It is based on the measure of the resistance of conjugates formed between T cells and adherent tumor cells to shear stress in a microfluidic system. For this purpose, T cells, stained with a CellTracker probe, are added into flow channels containing a monolayer of adherent target cells and their progressive detachment under a constant shear stress is then recorded using a fluorescent microscope.
[摘要] 该方法允许使用流动室中的剪切力评估T淋巴细胞和特异性粘附靶细胞之间的相对粘附强度。 其基于在微流体系统中T细胞和粘附的肿瘤细胞之间形成的缀合物对剪切应力的抗性的测量。 为此，将用CellTracker探针染色的T细胞加入含有单层粘附靶细胞的流动通道中，然后使用荧光显微镜记录在恒定剪切应力下的其逐渐分离。
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